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Al-Hanbali Maysa; Al, " Epicatechin suppresses IL-6, IL-8 and enhances IL-10 production with NF-?B nuclear translocation in whole blood stimulated system " , "Neuroendrocrinology Letters",Vol.30,No.1, SOCIETY OF INTEGRATED SCIENCES, Amman-Jordan, 06/08/2009 Abstract:
OBJECTIVES: Cytokines induce the balance between inflammatory versus regulatory or antibody mediated reactions. So modulating the release of cytokines or inducing them by immunomodulating agents is an attractive mode for treating or help in treating several diseases such as autoimmune diseases. Eriobotrya japonica is a plant that is traditionally thought to have anti-inflammatory activities. Several compounds were isolated from the plant and showed distinctive biological effects. The purpose of this study was to determine the effects of epicatechin (EC) isolated from Eriobotrya Japonica on IL-6, IL-8, and IL-10 productions in whole blood stimulated with phytohemagglutinin (PHA)+lipopolysaccharide (LPS), and to examine if these cytokines are modulated through NFkappaB pathway. METHODS: Sixteen healthy males and females volunteered in the study. Blood samples were drawn, diluted, and cultured for 24 h with different concentrations of EC and then PHA+LPS was added for another 24 h. The supernatant, then, was harvested and assayed for cytokines. In addition, mixing studies of EC and hydrocortisone were performed to examine the cytoplasmic and nuclear fractions of NFkB levels in association with cytokine production levels. RESULTS: Increasing concentrations of EC (1-100 microg/ml) in PHA+LPS stimulated whole blood cells culture suppressed significantly (p<0.001) the production of IL-6 and IL-8. Moreover, increasing concentrations of EC modulated significantly the production of IL-10, as there was a significant increase in IL-10 level at 0.1, 1.0, and 10 microg/ml (p=0.058-0.004), while a significant decrease at a concentration of 100 microg/ml EC (p=0.037) was observed. In addition, an additive effect between EC and hydrocortisone (HC, 100 nmol/l) was seen in the production of IL-10, as there was a significant increase in IL-10 level (32%) compared with 27% for EC (10 microg/ml) and 19% HC. Furthermore, a significant decrease in cytoplasmic fractions of NFkappaB p65 level was found in samples containing EC 1, 10 microg/ml, but not in 100 microg/ml, when compared with control (p<0.03). These latter changes were accompanied with a 29%, 67%, and 98% increase, respectively, of NFkappaB p65 in nuclear fractions compared to 24% reduction of NFkappaB p65 level in HC culture (p<0.03). CONCLUSIONS: These results indicate that EC suppresses the production of pro-inflammatory cytokines, IL6 and IL-8, enhances the production of anti-inflammatory cytokine, IL-10, and stimulates NFkappaB p65 translocation to nucleus in PHA+LPS stimulated whole blood culture. Download




Hantash Abed El-lal, " Mec A and PVL genes in Methicillin resistant Staphylococcus aureus from nasal carrier individuals exposed and not exposed to hospital environments " , "Arab Journal of Pharmaceutical Sciences ",Vol.3,No.9, Philadelphia University, Amman - Jordan, 06/07/2009 Abstract:
Mec A and PVL genes in Methicillin resistant Staphylococcus aureus from nasal carrier individuals exposed and not exposed to hospital environments Download




Heba A Alshaker, Nid, " Eriobotrya japonica hydrophilic extract modulates cytokines in normal tissues, in the tumor of Meth-A-fibrosarcoma bearing mice, and enhances their survival time " , "complementary and alternative Medicine ",Vol.11,No.9, Biomed Central, Amman - Jordan, 02/04/2011 Abstract:
BACKGROUND: Cytokines play a key role in the immune response to developing tumors, and therefore modulating their levels and actions provides innovative strategies for enhancing the activity of antigen presenting cells and polarizing towards T helper 1 type response within tumor microenvironment. One of these approaches could be the employment of plant extracts that have cytokine immunomodulation capabilities. Previously, we have shown that the Eriobotrya japonica hydrophilic extract (EJHE) induces proinflammatory cytokines in vitro and in vivo. METHODS: The present study explored the in vivo immunomodulatory effect on interferon-gamma (IFN-γ), interleukin-17 (IL-17), and transforming growth factor-beta 1 (TGF-β1) evoked by two water-extracts prepared from EJ leaves in the tissues of normal and Meth-A-fibrosarcoma bearing mice. RESULTS: Intraperitoneal (i.p.) administration of 10 μg of EJHE and EJHE-water residue (WR), prepared from butanol extraction, increased significantly IFN-γ production in the spleen (p < 0.01) and lung (p < 0.03) tissues at 6-48 hours and suppressed significantly TGF-β1 production levels (p < 0.001) in the spleen for as long as 48 hours. The latter responses, however, were not seen in Meth-A fibrosarcoma-bearing mice. On the contrary, triple i.p. injections, 24 hours apart; of 10 μg EJHE increased significantly IFN-γ production in the spleen (p < 0.02) while only EJHE-WR increased significantly IFN-γ, TGF-β1 and IL-17 (p < 0.03 - 0.005) production within the tumor microenvironment of Meth-A fibrosarcoma. In addition, the present work revealed a significant prolongation of survival time (median survival time 72 days vs. 27 days of control, p < 0.007) of mice inoculated i.p. with Meth-A cells followed by three times/week for eight weeks of i.p. administration of EJHE-WR. The latter prolonged survival effect was not seen with EJHE. CONCLUSIONS: The therapeutic value of EJHE-WR as an anticancer agent merits further investigation of understanding the effect of immunomodulators' constituents on the cellular components of the tissue microenvironment. This can lead to the development of improved strategies for cancer treatment and thus opening up a new frontier for future studies. Download




Raida K., Mai K., Ma, " Assessment of the preserving efficacy of the pharmaceutical Syrups to Identified Air-Borne Microorganisms " , "Trends Journal of Applied Sciences",Vol.6,No.2, Academic Journals Inc., USA, Amman-Jordan, 05/05/2011 Abstract:
The purpose of the current study was a continuation of the evaluation of the efficacy of different preservatives ingredients of different cough syrups and which previously assessed to Air-borne microorganisms. The airborne microorganisms were characterized firstly according to their colony color and then to their DNA sequences. The identified microorganisms were inoculated into syrup A (glycerol and propylene glycol), syrup B (propylene glycol and glycerin), syrup (C) (glycerin, propylene glycol and butyl paraben), syrup D (methyl paraben and propyl paraben) and normal saline as a control which were then incubated for 24 h. Growth of microorganisms into syrup was compared by counting the CFUs from a subculture of inoculated syrup at different time. The data showed that the genome of the three isolated air-borne microbes revealed three different species and that all the combinations of the preservatives in the four studied cough syrups behaved similarly in term of efficacy towards the microbial contamination. The results revealed that the preservatives mixtures of propylene glycol with glycerol or with glycerin or with butyl paraben preservatives and the methyl paraben with propyl paraben are have efficient antimicrobial activity against Airborne microbes during the 24 h studied period. Download




Mona R. Bustami, Nermen Dakkak Lauy F. Abu-Qatouseh Raida Khalil Khalid Z. Matalka, " Value of phenotyic and genotypic identification of Acinetobacter baumannii isolates from two hospitals in Jordan " , "The International Arabic Journal of Antimicrobial Agents",Vol.,No., http://www.imed.pub, , 02/10/2016 Abstract:
Acinetobacter baumannii isolates have been recovered from hospitalized patients over the past few years from two hospitals in Jordan. Phenotypic and biochemical characterization in addition to disc susceptibility testing of all clinical isolates indicated that all of them were belonging to A. bauman Download




W. Abu Rayyan, A. Singh, A. M. Al-Jaafreh, W. Abu Dayyih, M. Bustami, S. Salem, N. Seder, K. Schr?ppel, " The Role of Glutamine-Rich Region of Candida Albicans Tec1p in Mediating Morphological Transition and Invasive Growth " , "International Journal of Medical and Health Sciences",Vol.13,No., Octa publishing, India, 02/03/2019 Abstract:
Hyphal growth and the transcriptional regulation to the host environment are key issues during the pathogenesis of C. albicans. Tec1p is the C. albicans homolog of a TEA transcription factor family, which share a conserved DNA-binding TEA domain in their N-terminal. In order to define a structure-fu Download




Mona Bustami, Khalid Z Matalka, Eyad Mallah, Luay Abu-Qatouseh, Wael Abu Dayyih, Nour Hussein, Nayef Abu Safieh, Yousef Elyyan, Nagham Hussein, Tawfiq Arafat, " The Prevalence of Overweight and Obesity Among Women in Jordan: A Risk Factor for Developing Chronic Diseases " , "Journal of Multidisciplinary Healthcare",Vol.14,No., Dove Medical press, UNITED KINGDOM, 06/22/2021 Abstract:
The study aimed to investigate the prevalence of obesity among Jordanian women and its association with a wide range of chronic diseases. There is a high prevalence of overweight/obesity among women in Jordan, which was related to high parity and low education level. This high prevalence of obesity Download